High seroprevalence for SARS-CoV-2 among household members of essential workers detected using a dried blood spot assay.
Identifieur interne : 000540 ( Main/Exploration ); précédent : 000539; suivant : 000541High seroprevalence for SARS-CoV-2 among household members of essential workers detected using a dried blood spot assay.
Auteurs : Thomas W. Mcdade [États-Unis, Canada] ; Elizabeth M. Mcnally [États-Unis] ; Aaron S. Zelikovich [États-Unis] ; Richard D'Aquila [États-Unis] ; Brian Mustanski [États-Unis] ; Aaron Miller [États-Unis] ; Lauren A. Vaught [États-Unis] ; Nina L. Reiser [États-Unis] ; Elena Bogdanovic [États-Unis] ; Katherine S. Fallon [États-Unis] ; Alexis R. Demonbreun [États-Unis]Source :
- PloS one [ 1932-6203 ] ; 2020.
Descripteurs français
- KwdFr :
- Adolescent (MeSH), Adulte (MeSH), Adulte d'âge moyen (MeSH), Anticorps antiviraux (sang), Betacoronavirus (immunologie), Caractéristiques familiales (MeSH), Dépistage sur goutte de sang séché (MeSH), Femelle (MeSH), Glycoprotéine de spicule des coronavirus (immunologie), Humains (MeSH), Immunoglobuline G (sang), Infections à coronavirus (diagnostic), Infections à coronavirus (transmission), Infections à coronavirus (virologie), Infections à coronavirus (épidémiologie), Jeune adulte (MeSH), Mâle (MeSH), Pandémies (MeSH), Personnel de santé (MeSH), Pneumopathie virale (diagnostic), Pneumopathie virale (transmission), Pneumopathie virale (virologie), Pneumopathie virale (épidémiologie), Sujet âgé (MeSH), Test ELISA (MeSH), Tests sérologiques (méthodes), Études séroépidémiologiques (MeSH).
- MESH :
- diagnostic : Infections à coronavirus, Pneumopathie virale.
- immunologie : Betacoronavirus, Glycoprotéine de spicule des coronavirus.
- méthodes : Tests sérologiques.
- sang : Anticorps antiviraux, Immunoglobuline G.
- virologie : Infections à coronavirus, Pneumopathie virale.
- épidémiologie : Infections à coronavirus, Pneumopathie virale.
- Adolescent, Adulte, Adulte d'âge moyen, Caractéristiques familiales, Dépistage sur goutte de sang séché, Femelle, Humains, Jeune adulte, Mâle, Pandémies, Personnel de santé, Sujet âgé, Test ELISA, Études séroépidémiologiques.
English descriptors
- KwdEn :
- Adolescent (MeSH), Adult (MeSH), Aged (MeSH), Antibodies, Viral (blood), Betacoronavirus (immunology), Coronavirus Infections (diagnosis), Coronavirus Infections (epidemiology), Coronavirus Infections (transmission), Coronavirus Infections (virology), Dried Blood Spot Testing (MeSH), Enzyme-Linked Immunosorbent Assay (MeSH), Family Characteristics (MeSH), Female (MeSH), Health Personnel (MeSH), Humans (MeSH), Immunoglobulin G (blood), Male (MeSH), Middle Aged (MeSH), Pandemics (MeSH), Pneumonia, Viral (diagnosis), Pneumonia, Viral (epidemiology), Pneumonia, Viral (transmission), Pneumonia, Viral (virology), Seroepidemiologic Studies (MeSH), Serologic Tests (methods), Spike Glycoprotein, Coronavirus (immunology), Young Adult (MeSH).
- MESH :
- chemical , blood : Antibodies, Viral, Immunoglobulin G.
- diagnosis : Coronavirus Infections, Pneumonia, Viral.
- epidemiology : Coronavirus Infections, Pneumonia, Viral.
- immunology : Betacoronavirus, Spike Glycoprotein, Coronavirus.
- methods : Serologic Tests.
- transmission : Coronavirus Infections, Pneumonia, Viral.
- virology : Coronavirus Infections, Pneumonia, Viral.
- Adolescent, Adult, Aged, Dried Blood Spot Testing, Enzyme-Linked Immunosorbent Assay, Family Characteristics, Female, Health Personnel, Humans, Male, Middle Aged, Pandemics, Seroepidemiologic Studies, Young Adult.
Abstract
OBJECTIVE
Serological testing is needed to investigate the extent of transmission of SARS-CoV-2 from front-line essential workers to their household members. However, the requirement for serum/plasma limits serological testing to clinical settings where it is feasible to collect and process venous blood. To address this problem we developed a serological test for SARS-CoV-2 IgG antibodies that requires only a single drop of finger stick capillary whole blood, collected in the home and dried on filter paper (dried blood spot, DBS). We describe assay performance and demonstrate its utility for remote sampling with results from a community-based study.
METHODS
An ELISA to the receptor binding domain of the SARS-CoV-2 spike protein was optimized to quantify IgG antibodies in DBS. Samples were self-collected from a community sample of 232 participants enriched with health care workers, including 30 known COVID-19 cases and their household members.
RESULTS
Among 30 individuals sharing a household with a virus-confirmed case of COVID-19, 80% were seropositive. Of 202 community individuals without prior confirmed acute COVID-19 diagnoses, 36% were seropositive. Of documented convalescent COVID-19 cases from the community, 29 of 30 (97%) were seropositive for IgG antibodies to the receptor binding domain.
CONCLUSION
DBS ELISA provides a minimally-invasive alternative to venous blood collection. Early analysis suggests a high rate of transmission among household members. High rates of seroconversion were also noted following recovery from infection. Serological testing for SARS-CoV-2 IgG antibodies in DBS samples can facilitate seroprevalence assessment in community settings to address epidemiological questions, monitor duration of antibody responses, and assess if antibodies against the spike protein correlate with protection from reinfection.
DOI: 10.1371/journal.pone.0237833
PubMed: 32797108
PubMed Central: PMC7428174
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Mcdade</name><affiliation wicri:level="2"><nlm:affiliation>Department of Anthropology and Institute for Policy Research, Northwestern University, Evanston, Illinois, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Anthropology and Institute for Policy Research, Northwestern University, Evanston, Illinois</wicri:regionArea><placeName><region type="state">Illinois</region></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Canadian Institute for Advanced Research, Child and Brain Development Program, Toronto, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Canadian Institute for Advanced Research, Child and Brain Development Program, Toronto</wicri:regionArea><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName></affiliation></author><author><name sortKey="Mcnally, Elizabeth M" sort="Mcnally, Elizabeth M" uniqKey="Mcnally E" first="Elizabeth M" last="Mcnally">Elizabeth M. Mcnally</name><affiliation wicri:level="2"><nlm:affiliation>Center for Genetic Medicine, Northwestern University, Evanston, Illinois, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Genetic Medicine, Northwestern University, Evanston, Illinois</wicri:regionArea><placeName><region type="state">Illinois</region></placeName></affiliation><affiliation wicri:level="2"><nlm:affiliation>Division of Cardiology, Department of Medicine, Northwestern University Feinberg School of Medicine, Chicago, Illinois, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Division of Cardiology, Department of Medicine, Northwestern University Feinberg School of Medicine, Chicago, Illinois</wicri:regionArea><placeName><region type="state">Illinois</region></placeName></affiliation><affiliation wicri:level="2"><nlm:affiliation>Department of Biochemistry and Molecular Genetics, Northwestern University, Evanston, Illinois, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biochemistry and Molecular Genetics, Northwestern University, Evanston, Illinois</wicri:regionArea><placeName><region type="state">Illinois</region></placeName></affiliation></author><author><name sortKey="Zelikovich, Aaron S" sort="Zelikovich, Aaron S" uniqKey="Zelikovich A" first="Aaron S" last="Zelikovich">Aaron S. Zelikovich</name><affiliation wicri:level="2"><nlm:affiliation>Center for Genetic Medicine, Northwestern University, Evanston, Illinois, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Genetic Medicine, Northwestern University, Evanston, Illinois</wicri:regionArea><placeName><region type="state">Illinois</region></placeName></affiliation><affiliation wicri:level="2"><nlm:affiliation>Division of Cardiology, Department of Medicine, Northwestern University Feinberg School of Medicine, Chicago, Illinois, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Division of Cardiology, Department of Medicine, Northwestern University Feinberg School of Medicine, Chicago, Illinois</wicri:regionArea><placeName><region type="state">Illinois</region></placeName></affiliation></author><author><name sortKey="D Aquila, Richard" sort="D Aquila, Richard" uniqKey="D Aquila R" first="Richard" last="D'Aquila">Richard D'Aquila</name><affiliation wicri:level="2"><nlm:affiliation>Division of Infectious Diseases, Department of Medicine, Northwestern University Feinberg School of Medicine, Chicago, Illinois, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Division of Infectious Diseases, Department of Medicine, Northwestern University Feinberg School of Medicine, Chicago, Illinois</wicri:regionArea><placeName><region type="state">Illinois</region></placeName></affiliation></author><author><name sortKey="Mustanski, Brian" sort="Mustanski, Brian" uniqKey="Mustanski B" first="Brian" last="Mustanski">Brian Mustanski</name><affiliation wicri:level="2"><nlm:affiliation>Institute for Sexual and Gender Minority Health and Wellbeing and Department of Medical Social Sciences, Northwestern University, Chicago, Illinois, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Institute for Sexual and Gender Minority Health and Wellbeing and Department of Medical Social Sciences, Northwestern University, Chicago, Illinois</wicri:regionArea><placeName><region type="state">Illinois</region></placeName></affiliation></author><author><name sortKey="Miller, Aaron" sort="Miller, Aaron" uniqKey="Miller A" first="Aaron" last="Miller">Aaron Miller</name><affiliation wicri:level="2"><nlm:affiliation>Department of Anthropology and Institute for Policy Research, Northwestern University, Evanston, Illinois, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Anthropology and Institute for Policy Research, Northwestern University, Evanston, Illinois</wicri:regionArea><placeName><region type="state">Illinois</region></placeName></affiliation></author><author><name sortKey="Vaught, Lauren A" sort="Vaught, Lauren A" uniqKey="Vaught L" first="Lauren A" last="Vaught">Lauren A. Vaught</name><affiliation wicri:level="2"><nlm:affiliation>Center for Genetic Medicine, Northwestern University, Evanston, Illinois, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Genetic Medicine, Northwestern University, Evanston, Illinois</wicri:regionArea><placeName><region type="state">Illinois</region></placeName></affiliation><affiliation wicri:level="2"><nlm:affiliation>Division of Cardiology, Department of Medicine, Northwestern University Feinberg School of Medicine, Chicago, Illinois, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Division of Cardiology, Department of Medicine, Northwestern University Feinberg School of Medicine, Chicago, Illinois</wicri:regionArea><placeName><region type="state">Illinois</region></placeName></affiliation></author><author><name sortKey="Reiser, Nina L" sort="Reiser, Nina L" uniqKey="Reiser N" first="Nina L" last="Reiser">Nina L. Reiser</name><affiliation wicri:level="2"><nlm:affiliation>Center for Genetic Medicine, Northwestern University, Evanston, Illinois, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Genetic Medicine, Northwestern University, Evanston, Illinois</wicri:regionArea><placeName><region type="state">Illinois</region></placeName></affiliation><affiliation wicri:level="2"><nlm:affiliation>Division of Cardiology, Department of Medicine, Northwestern University Feinberg School of Medicine, Chicago, Illinois, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Division of Cardiology, Department of Medicine, Northwestern University Feinberg School of Medicine, Chicago, Illinois</wicri:regionArea><placeName><region type="state">Illinois</region></placeName></affiliation></author><author><name sortKey="Bogdanovic, Elena" sort="Bogdanovic, Elena" uniqKey="Bogdanovic E" first="Elena" last="Bogdanovic">Elena Bogdanovic</name><affiliation wicri:level="2"><nlm:affiliation>Center for Genetic Medicine, Northwestern University, Evanston, Illinois, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Genetic Medicine, Northwestern University, Evanston, Illinois</wicri:regionArea><placeName><region type="state">Illinois</region></placeName></affiliation><affiliation wicri:level="2"><nlm:affiliation>Division of Cardiology, Department of Medicine, Northwestern University Feinberg School of Medicine, Chicago, Illinois, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Division of Cardiology, Department of Medicine, Northwestern University Feinberg School of Medicine, Chicago, Illinois</wicri:regionArea><placeName><region type="state">Illinois</region></placeName></affiliation></author><author><name sortKey="Fallon, Katherine S" sort="Fallon, Katherine S" uniqKey="Fallon K" first="Katherine S" last="Fallon">Katherine S. Fallon</name><affiliation wicri:level="2"><nlm:affiliation>Center for Genetic Medicine, Northwestern University, Evanston, Illinois, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Genetic Medicine, Northwestern University, Evanston, Illinois</wicri:regionArea><placeName><region type="state">Illinois</region></placeName></affiliation><affiliation wicri:level="2"><nlm:affiliation>Division of Cardiology, Department of Medicine, Northwestern University Feinberg School of Medicine, Chicago, Illinois, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Division of Cardiology, Department of Medicine, Northwestern University Feinberg School of Medicine, Chicago, Illinois</wicri:regionArea><placeName><region type="state">Illinois</region></placeName></affiliation></author><author><name sortKey="Demonbreun, Alexis R" sort="Demonbreun, Alexis R" uniqKey="Demonbreun A" first="Alexis R" last="Demonbreun">Alexis R. Demonbreun</name><affiliation wicri:level="2"><nlm:affiliation>Center for Genetic Medicine, Northwestern University, Evanston, Illinois, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Genetic Medicine, Northwestern University, Evanston, Illinois</wicri:regionArea><placeName><region type="state">Illinois</region></placeName></affiliation><affiliation wicri:level="2"><nlm:affiliation>Department of Pharmacology, Northwestern University Feinberg School of Medicine, Chicago, Illinois, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pharmacology, Northwestern University Feinberg School of Medicine, Chicago, Illinois</wicri:regionArea><placeName><region type="state">Illinois</region></placeName></affiliation></author></analytic><series><title level="j">PloS one</title><idno type="eISSN">1932-6203</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adolescent (MeSH)</term><term>Adult (MeSH)</term><term>Aged (MeSH)</term><term>Antibodies, Viral (blood)</term><term>Betacoronavirus (immunology)</term><term>Coronavirus Infections (diagnosis)</term><term>Coronavirus Infections (epidemiology)</term><term>Coronavirus Infections (transmission)</term><term>Coronavirus Infections (virology)</term><term>Dried Blood Spot Testing (MeSH)</term><term>Enzyme-Linked Immunosorbent Assay (MeSH)</term><term>Family Characteristics (MeSH)</term><term>Female (MeSH)</term><term>Health Personnel (MeSH)</term><term>Humans (MeSH)</term><term>Immunoglobulin G (blood)</term><term>Male (MeSH)</term><term>Middle Aged (MeSH)</term><term>Pandemics (MeSH)</term><term>Pneumonia, Viral (diagnosis)</term><term>Pneumonia, Viral (epidemiology)</term><term>Pneumonia, Viral (transmission)</term><term>Pneumonia, Viral (virology)</term><term>Seroepidemiologic Studies (MeSH)</term><term>Serologic Tests (methods)</term><term>Spike Glycoprotein, Coronavirus (immunology)</term><term>Young Adult (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adolescent (MeSH)</term><term>Adulte (MeSH)</term><term>Adulte d'âge moyen (MeSH)</term><term>Anticorps antiviraux (sang)</term><term>Betacoronavirus (immunologie)</term><term>Caractéristiques familiales (MeSH)</term><term>Dépistage sur goutte de sang séché (MeSH)</term><term>Femelle (MeSH)</term><term>Glycoprotéine de spicule des coronavirus (immunologie)</term><term>Humains (MeSH)</term><term>Immunoglobuline G (sang)</term><term>Infections à coronavirus (diagnostic)</term><term>Infections à coronavirus (transmission)</term><term>Infections à coronavirus (virologie)</term><term>Infections à coronavirus (épidémiologie)</term><term>Jeune adulte (MeSH)</term><term>Mâle (MeSH)</term><term>Pandémies (MeSH)</term><term>Personnel de santé (MeSH)</term><term>Pneumopathie virale (diagnostic)</term><term>Pneumopathie virale (transmission)</term><term>Pneumopathie virale (virologie)</term><term>Pneumopathie virale (épidémiologie)</term><term>Sujet âgé (MeSH)</term><term>Test ELISA (MeSH)</term><term>Tests sérologiques (méthodes)</term><term>Études séroépidémiologiques (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Viral</term><term>Immunoglobulin G</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Coronavirus Infections</term><term>Pneumonia, Viral</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Infections à coronavirus</term><term>Pneumopathie virale</term></keywords><keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Coronavirus Infections</term><term>Pneumonia, Viral</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Betacoronavirus</term><term>Glycoprotéine de spicule des coronavirus</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Betacoronavirus</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Serologic Tests</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Tests sérologiques</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antiviraux</term><term>Immunoglobuline G</term></keywords><keywords scheme="MESH" qualifier="transmission" xml:lang="en"><term>Coronavirus Infections</term><term>Pneumonia, Viral</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Infections à coronavirus</term><term>Pneumopathie virale</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Coronavirus Infections</term><term>Pneumonia, Viral</term></keywords><keywords scheme="MESH" qualifier="épidémiologie" xml:lang="fr"><term>Infections à coronavirus</term><term>Pneumopathie virale</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adolescent</term><term>Adult</term><term>Aged</term><term>Dried Blood Spot Testing</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Family Characteristics</term><term>Female</term><term>Health Personnel</term><term>Humans</term><term>Male</term><term>Middle Aged</term><term>Pandemics</term><term>Seroepidemiologic Studies</term><term>Young Adult</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adolescent</term><term>Adulte</term><term>Adulte d'âge moyen</term><term>Caractéristiques familiales</term><term>Dépistage sur goutte de sang séché</term><term>Femelle</term><term>Humains</term><term>Jeune adulte</term><term>Mâle</term><term>Pandémies</term><term>Personnel de santé</term><term>Sujet âgé</term><term>Test ELISA</term><term>Études séroépidémiologiques</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>OBJECTIVE</b></p><p>Serological testing is needed to investigate the extent of transmission of SARS-CoV-2 from front-line essential workers to their household members. However, the requirement for serum/plasma limits serological testing to clinical settings where it is feasible to collect and process venous blood. To address this problem we developed a serological test for SARS-CoV-2 IgG antibodies that requires only a single drop of finger stick capillary whole blood, collected in the home and dried on filter paper (dried blood spot, DBS). We describe assay performance and demonstrate its utility for remote sampling with results from a community-based study.</p></div><div type="abstract" xml:lang="en"><p><b>METHODS</b></p><p>An ELISA to the receptor binding domain of the SARS-CoV-2 spike protein was optimized to quantify IgG antibodies in DBS. Samples were self-collected from a community sample of 232 participants enriched with health care workers, including 30 known COVID-19 cases and their household members.</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>Among 30 individuals sharing a household with a virus-confirmed case of COVID-19, 80% were seropositive. Of 202 community individuals without prior confirmed acute COVID-19 diagnoses, 36% were seropositive. Of documented convalescent COVID-19 cases from the community, 29 of 30 (97%) were seropositive for IgG antibodies to the receptor binding domain.</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSION</b></p><p>DBS ELISA provides a minimally-invasive alternative to venous blood collection. Early analysis suggests a high rate of transmission among household members. High rates of seroconversion were also noted following recovery from infection. Serological testing for SARS-CoV-2 IgG antibodies in DBS samples can facilitate seroprevalence assessment in community settings to address epidemiological questions, monitor duration of antibody responses, and assess if antibodies against the spike protein correlate with protection from reinfection.</p></div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">32797108</PMID><DateCompleted><Year>2020</Year><Month>08</Month><Day>28</Day></DateCompleted><DateRevised><Year>2020</Year><Month>08</Month><Day>28</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Electronic">1932-6203</ISSN><JournalIssue CitedMedium="Internet"><Volume>15</Volume><Issue>8</Issue><PubDate><Year>2020</Year></PubDate></JournalIssue><Title>PloS one</Title><ISOAbbreviation>PLoS One</ISOAbbreviation></Journal><ArticleTitle>High seroprevalence for SARS-CoV-2 among household members of essential workers detected using a dried blood spot assay.</ArticleTitle><Pagination><MedlinePgn>e0237833</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1371/journal.pone.0237833</ELocationID><Abstract><AbstractText Label="OBJECTIVE">Serological testing is needed to investigate the extent of transmission of SARS-CoV-2 from front-line essential workers to their household members. However, the requirement for serum/plasma limits serological testing to clinical settings where it is feasible to collect and process venous blood. To address this problem we developed a serological test for SARS-CoV-2 IgG antibodies that requires only a single drop of finger stick capillary whole blood, collected in the home and dried on filter paper (dried blood spot, DBS). We describe assay performance and demonstrate its utility for remote sampling with results from a community-based study.</AbstractText><AbstractText Label="METHODS">An ELISA to the receptor binding domain of the SARS-CoV-2 spike protein was optimized to quantify IgG antibodies in DBS. Samples were self-collected from a community sample of 232 participants enriched with health care workers, including 30 known COVID-19 cases and their household members.</AbstractText><AbstractText Label="RESULTS">Among 30 individuals sharing a household with a virus-confirmed case of COVID-19, 80% were seropositive. Of 202 community individuals without prior confirmed acute COVID-19 diagnoses, 36% were seropositive. Of documented convalescent COVID-19 cases from the community, 29 of 30 (97%) were seropositive for IgG antibodies to the receptor binding domain.</AbstractText><AbstractText Label="CONCLUSION">DBS ELISA provides a minimally-invasive alternative to venous blood collection. Early analysis suggests a high rate of transmission among household members. High rates of seroconversion were also noted following recovery from infection. Serological testing for SARS-CoV-2 IgG antibodies in DBS samples can facilitate seroprevalence assessment in community settings to address epidemiological questions, monitor duration of antibody responses, and assess if antibodies against the spike protein correlate with protection from reinfection.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>McDade</LastName><ForeName>Thomas W</ForeName><Initials>TW</Initials><Identifier Source="ORCID">0000-0001-5829-648X</Identifier><AffiliationInfo><Affiliation>Department of Anthropology and Institute for Policy Research, Northwestern University, Evanston, Illinois, United States of America.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Canadian Institute for Advanced Research, Child and Brain Development Program, Toronto, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>McNally</LastName><ForeName>Elizabeth M</ForeName><Initials>EM</Initials><AffiliationInfo><Affiliation>Center for Genetic Medicine, Northwestern University, Evanston, Illinois, United States of America.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Division of Cardiology, Department of Medicine, Northwestern University Feinberg School of Medicine, Chicago, Illinois, United States of America.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Biochemistry and Molecular Genetics, Northwestern University, Evanston, Illinois, United States of America.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zelikovich</LastName><ForeName>Aaron S</ForeName><Initials>AS</Initials><AffiliationInfo><Affiliation>Center for Genetic Medicine, Northwestern University, Evanston, Illinois, United States of America.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Division of Cardiology, Department of Medicine, Northwestern University Feinberg School of Medicine, Chicago, Illinois, United States of America.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>D'Aquila</LastName><ForeName>Richard</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Division of Infectious Diseases, Department of Medicine, Northwestern University Feinberg School of Medicine, Chicago, Illinois, United States of America.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mustanski</LastName><ForeName>Brian</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Institute for Sexual and Gender Minority Health and Wellbeing and Department of Medical Social Sciences, Northwestern University, Chicago, Illinois, United States of America.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Miller</LastName><ForeName>Aaron</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Department of Anthropology and Institute for Policy Research, Northwestern University, Evanston, Illinois, United States of America.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Vaught</LastName><ForeName>Lauren A</ForeName><Initials>LA</Initials><Identifier Source="ORCID">0000-0001-8634-9139</Identifier><AffiliationInfo><Affiliation>Center for Genetic Medicine, Northwestern University, Evanston, Illinois, United States of America.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Division of Cardiology, Department of Medicine, Northwestern University Feinberg School of Medicine, Chicago, Illinois, United States of America.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Reiser</LastName><ForeName>Nina L</ForeName><Initials>NL</Initials><Identifier Source="ORCID">0000-0002-4331-163X</Identifier><AffiliationInfo><Affiliation>Center for Genetic Medicine, Northwestern University, Evanston, Illinois, United States of America.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Division of Cardiology, Department of Medicine, Northwestern University Feinberg School of Medicine, Chicago, Illinois, United States of America.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bogdanovic</LastName><ForeName>Elena</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Center for Genetic Medicine, Northwestern University, Evanston, Illinois, United States of America.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Division of Cardiology, Department of Medicine, Northwestern University Feinberg School of Medicine, Chicago, Illinois, United States of America.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Fallon</LastName><ForeName>Katherine S</ForeName><Initials>KS</Initials><AffiliationInfo><Affiliation>Center for Genetic Medicine, Northwestern University, Evanston, Illinois, United States of America.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Division of Cardiology, Department of Medicine, Northwestern University Feinberg School of Medicine, Chicago, Illinois, United States of America.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Demonbreun</LastName><ForeName>Alexis R</ForeName><Initials>AR</Initials><AffiliationInfo><Affiliation>Center for Genetic Medicine, Northwestern University, Evanston, Illinois, United States of America.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Pharmacology, Northwestern University Feinberg School of Medicine, Chicago, Illinois, United States of America.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>08</Month><Day>14</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>PLoS One</MedlineTA><NlmUniqueID>101285081</NlmUniqueID><ISSNLinking>1932-6203</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000914">Antibodies, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007074">Immunoglobulin G</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D064370">Spike Glycoprotein, Coronavirus</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C000657845">spike protein, SARS-CoV-2</NameOfSubstance></Chemical></ChemicalList><SupplMeshList><SupplMeshName Type="Disease" UI="C000657245">COVID-19</SupplMeshName><SupplMeshName Type="Organism" UI="C000656484">severe acute respiratory syndrome coronavirus 2</SupplMeshName></SupplMeshList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000293" MajorTopicYN="N">Adolescent</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000328" MajorTopicYN="N">Adult</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000368" MajorTopicYN="N">Aged</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000914" MajorTopicYN="N">Antibodies, Viral</DescriptorName><QualifierName UI="Q000097" MajorTopicYN="N">blood</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000073640" MajorTopicYN="N">Betacoronavirus</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018352" MajorTopicYN="N">Coronavirus Infections</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="Y">diagnosis</QualifierName><QualifierName UI="Q000453" MajorTopicYN="Y">epidemiology</QualifierName><QualifierName UI="Q000635" MajorTopicYN="N">transmission</QualifierName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D059788" MajorTopicYN="Y">Dried Blood Spot Testing</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004797" MajorTopicYN="N">Enzyme-Linked Immunosorbent Assay</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005191" MajorTopicYN="Y">Family Characteristics</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006282" MajorTopicYN="Y">Health Personnel</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007074" MajorTopicYN="N">Immunoglobulin G</DescriptorName><QualifierName UI="Q000097" MajorTopicYN="N">blood</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008875" MajorTopicYN="N">Middle Aged</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D058873" MajorTopicYN="N">Pandemics</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011024" MajorTopicYN="N">Pneumonia, Viral</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="Y">diagnosis</QualifierName><QualifierName UI="Q000453" MajorTopicYN="Y">epidemiology</QualifierName><QualifierName UI="Q000635" MajorTopicYN="N">transmission</QualifierName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016036" MajorTopicYN="N">Seroepidemiologic Studies</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012698" MajorTopicYN="N">Serologic Tests</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D064370" MajorTopicYN="N">Spike Glycoprotein, Coronavirus</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D055815" MajorTopicYN="N">Young Adult</DescriptorName></MeshHeading></MeshHeadingList><CoiStatement>The authors of this paper have read the journal's policy and have the following competing interests: Thomas W. McDade has a financial interest in EnMed Microanalytics, a company that performs laboratory tests using dried blood spot samples. There are no patents, products in development or marketed products associated with this research to declare. This does not alter our adherence to PLOS ONE policies on sharing data and materials.</CoiStatement></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>06</Month><Day>05</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>08</Month><Day>04</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>8</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>8</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>8</Month><Day>29</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32797108</ArticleId><ArticleId IdType="doi">10.1371/journal.pone.0237833</ArticleId><ArticleId IdType="pii">PONE-D-20-17116</ArticleId><ArticleId IdType="pmc">PMC7428174</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Psychosom Med. 2000 Jul-Aug;62(4):560-7</Citation><ArticleIdList><ArticleId IdType="pubmed">10949102</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2020 Jul;583(7815):290-295</Citation><ArticleIdList><ArticleId IdType="pubmed">32422645</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Med. 2020 Jul;26(7):1033-1036</Citation><ArticleIdList><ArticleId IdType="pubmed">32398876</ArticleId></ArticleIdList></Reference><Reference><Citation>J Clin Microbiol. 2020 Jul 23;58(8):</Citation><ArticleIdList><ArticleId IdType="pubmed">32381641</ArticleId></ArticleIdList></Reference><Reference><Citation>JAMA. 2020 May 18;:</Citation><ArticleIdList><ArticleId IdType="pubmed">32421144</ArticleId></ArticleIdList></Reference><Reference><Citation>JAMA. 2020 May 14;:</Citation><ArticleIdList><ArticleId IdType="pubmed">32407440</ArticleId></ArticleIdList></Reference><Reference><Citation>Clin Chem. 2018 Apr;64(4):656-679</Citation><ArticleIdList><ArticleId IdType="pubmed">29187355</ArticleId></ArticleIdList></Reference><Reference><Citation>Demography. 2007 Nov;44(4):899-925</Citation><ArticleIdList><ArticleId IdType="pubmed">18232218</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Canada</li><li>États-Unis</li></country><region><li>Illinois</li><li>Ontario</li></region><settlement><li>Toronto</li></settlement></list><tree><country name="États-Unis"><region name="Illinois"><name sortKey="Mcdade, Thomas W" sort="Mcdade, Thomas W" uniqKey="Mcdade T" first="Thomas W" last="Mcdade">Thomas W. Mcdade</name></region><name sortKey="Bogdanovic, Elena" sort="Bogdanovic, Elena" uniqKey="Bogdanovic E" first="Elena" last="Bogdanovic">Elena Bogdanovic</name><name sortKey="Bogdanovic, Elena" sort="Bogdanovic, Elena" uniqKey="Bogdanovic E" first="Elena" last="Bogdanovic">Elena Bogdanovic</name><name sortKey="D Aquila, Richard" sort="D Aquila, Richard" uniqKey="D Aquila R" first="Richard" last="D'Aquila">Richard D'Aquila</name><name sortKey="Demonbreun, Alexis R" sort="Demonbreun, Alexis R" uniqKey="Demonbreun A" first="Alexis R" last="Demonbreun">Alexis R. Demonbreun</name><name sortKey="Demonbreun, Alexis R" sort="Demonbreun, Alexis R" uniqKey="Demonbreun A" first="Alexis R" last="Demonbreun">Alexis R. Demonbreun</name><name sortKey="Fallon, Katherine S" sort="Fallon, Katherine S" uniqKey="Fallon K" first="Katherine S" last="Fallon">Katherine S. Fallon</name><name sortKey="Fallon, Katherine S" sort="Fallon, Katherine S" uniqKey="Fallon K" first="Katherine S" last="Fallon">Katherine S. Fallon</name><name sortKey="Mcnally, Elizabeth M" sort="Mcnally, Elizabeth M" uniqKey="Mcnally E" first="Elizabeth M" last="Mcnally">Elizabeth M. Mcnally</name><name sortKey="Mcnally, Elizabeth M" sort="Mcnally, Elizabeth M" uniqKey="Mcnally E" first="Elizabeth M" last="Mcnally">Elizabeth M. Mcnally</name><name sortKey="Mcnally, Elizabeth M" sort="Mcnally, Elizabeth M" uniqKey="Mcnally E" first="Elizabeth M" last="Mcnally">Elizabeth M. Mcnally</name><name sortKey="Miller, Aaron" sort="Miller, Aaron" uniqKey="Miller A" first="Aaron" last="Miller">Aaron Miller</name><name sortKey="Mustanski, Brian" sort="Mustanski, Brian" uniqKey="Mustanski B" first="Brian" last="Mustanski">Brian Mustanski</name><name sortKey="Reiser, Nina L" sort="Reiser, Nina L" uniqKey="Reiser N" first="Nina L" last="Reiser">Nina L. Reiser</name><name sortKey="Reiser, Nina L" sort="Reiser, Nina L" uniqKey="Reiser N" first="Nina L" last="Reiser">Nina L. Reiser</name><name sortKey="Vaught, Lauren A" sort="Vaught, Lauren A" uniqKey="Vaught L" first="Lauren A" last="Vaught">Lauren A. Vaught</name><name sortKey="Vaught, Lauren A" sort="Vaught, Lauren A" uniqKey="Vaught L" first="Lauren A" last="Vaught">Lauren A. Vaught</name><name sortKey="Zelikovich, Aaron S" sort="Zelikovich, Aaron S" uniqKey="Zelikovich A" first="Aaron S" last="Zelikovich">Aaron S. Zelikovich</name><name sortKey="Zelikovich, Aaron S" sort="Zelikovich, Aaron S" uniqKey="Zelikovich A" first="Aaron S" last="Zelikovich">Aaron S. Zelikovich</name></country><country name="Canada"><region name="Ontario"><name sortKey="Mcdade, Thomas W" sort="Mcdade, Thomas W" uniqKey="Mcdade T" first="Thomas W" last="Mcdade">Thomas W. Mcdade</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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